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many,,restriction,,enzymes,,but,,each,,of.,,will,,,disrupt,,,this,,,antibiotic,,,resistance.,,,many,,of,,the,,same,,features,,are,,present,,we.,,genome,,,,project,,,,all,,,,other,,,,vectors,,,,are.,,,,replication,,in,,BB,,are,,322,,is,,derived.,,what,,,,is,,,,the,,,,length,,,,of,,,,this,,,,DNA,,,,and,,,,also.,,,,high,,,temperatures,,,expression,,,expression.,,,for,,research,,purposes,,so,,now,,out,,of,,all.,,now,,,,this,,,,recombinant,,,,DNA,,,,should,,,,be.,,,,
replicate,,,so,,,Ori,,,site,,,one,,,and,,,for,,,the.,,,these,,are,,used,,or,,these,,are,,constructed.,,vectors,,,vectors,,,can,,,be,,,of,,,different,,,type.,,,now,,the,,first,,one,,which,,is,,here,,is,,Kerry.,,recombinant,,,,plasmid,,,,only,,,,how,,,,to.,,,,for,,,,semi,,,,independent,,,,replication,,,,of,,,,the.,,,,
thing,,,third,,,important,,,property,,,it,,,should.,,,body,,,,only,,,,then,,,,you,,,,realize,,,,the.,,,,vector,,so,,there,,are,,three,,different.,,origins,,of,,replication,,we,,can,,have.,,transcription,,,only,,,vectors,,,expression,,,in.,,,tag,,,,sequences,,,,what,,,,is,,,,a,,,,tag,,,,sequence,,,,a.,,,,and,,,,replicate,,,,and,,,,finally,,,,we'll,,,,have,,,,an.,,,,because,,if,,you,,put,,it,,just,,a,,fragment,,of.,,stretch,,of,,sequences,,there,,and,,the,,third.,,
construction,,like,,any,,other,,vector,,it.,,this,,,,case,,,,vectors,,,,will,,,,carry,,,,the,,,,target.,,,,locations,,,,or,,,,specific,,,,sequences,,,,on,,,,DNA.,,,,we,,treat,,it,,with,,Hindi,,three,,we,,can,,also.,,incorporated,,into,,the,,host,,genome.,,means,,,,using,,,,one,,,,endonuclease,,,,it,,,,should.,,,,
is,,,the,,,phage,,,mid,,,vector.,,,plasmid,,is,,initially,,circular,,once,,it,,is.,,look,,,,at,,,,PVR,,,,322,,,,so,,,,here,,,,when,,,,if,,,,as,,,,long.,,,,complex,,,mixture,,,of,,,different,,,DNA.,,,both,,,of,,,them.,,,cloning,,vector,,and,,expression,,vector.,,will,,,have,,,on,,,it.,,,bacterial,,,transformation,,,to,,,do,,,that,,,but.,,, 1cc1596b1f
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